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Assessing conservation of alternative splicing with evolutionary splicing graphs.

TitleAssessing conservation of alternative splicing with evolutionary splicing graphs.
Publication TypeJournal Article
Year of Publication2021
AuthorsZea, DJavier, Laskina, S, Baudin, A, Richard, H, Laine, E
JournalGenome Res
Date Published2021 Aug

Understanding how protein function has evolved and diversified is of great importance for human genetics and medicine. Here, we tackle the problem of describing the whole transcript variability observed in several species by generalizing the definition of splicing graph. We provide a practical solution to construct parsimonious evolutionary splicing graphs where each node is a minimal transcript building block defined across species. We show a clear link between the functional relevance, tissue regulation, and conservation of alternative transcripts on a set of 50 genes. By scaling up to the whole human protein-coding genome, we identify a few thousand genes where alternative splicing modulates the number and composition of pseudorepeats. We have implemented our approach in ThorAxe, an efficient, versatile, robust, and freely available computational tool.

Alternate JournalGenome Res
PubMed ID34266979
PubMed Central IDPMC8327911

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